Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
Waters hplc system cleaning procedure.
Label bottles correctly with bottle content and filling date expiry date.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
Disconnect the column and attach a union and flow directly to waste.
Residues or contaminations may block filters or capillaries.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
Maintaining your hplc uhplc uplc systems.
To clean the system.
When working with any lc system setting it up and storing it is very important.
Ion exchange to remove any remaining ions.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
Reverse osmosis to remove most contaminants b.
Use solvent inlet filters to protect the system from incoming particles.
Water flush to clean acid from the system.
Use only hplc grade solvents and water filtered through 0 2.
10 nh4oh in water this removes peg and other.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
Controlling contamination in lc ms and hplc ms systems 715001307.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
Remove all solvent filters.
Cleaning decontamination columns general guidelines flow cells general.
Responsibility quality control officer executive.
It is necessary to restore the system flow path to a near new level of cleanliness note.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
To clearly lay down the procedure for cleaning.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
Place a b c and d seal wash and needle wash lines into a clean.
The water flushes are only needed to remove the previous solvent from the system.
3 0 responsibility 3 1 doing.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
To clean the system.
No injections are needed with that.
